BIOCHEM QUESTION
Aminoacylase-1 is a dimeric with the structure a2. The total
mass is 90kDa. SDS-PAGE would show
a) 1 band at 180 kDa
b) 1 band at 90 kDa
c) 1 band at 45 kDa
d) 2 bands at 90 kDa

Answers

Answer 1

 The answer is d) 2 bands at 90 kDa.  

Aminoacylase-1 is a dimeric protein with a2 structure. The total mass of the aminoacylase-1 protein is 90 kDa. The electrophoresis technique called SDS-PAGE separates proteins according to their molecular weight. SDS is a negatively charged detergent that binds to proteins, causing them to denature and form a linear structure.  

When an electric field is applied to the mixture of proteins and SDS, the negatively charged proteins move towards the positive electrode. The migration of the proteins depends on their molecular weight.The answer is d) 2 bands at 90 kDa.  

The aminoacylase-1 protein is dimeric with an a2 structure. The molecular weight of a dimer is twice the weight of the monomer. Therefore, the molecular weight of aminoacylase-1 monomer is 90 kDa/2 = 45 kDa. SDS-PAGE would show two bands for a dimeric protein with a2 structure that has a total mass of 90 kDa.  

The two bands would be at the same position, and they would have a molecular weight of 90 kDa because the two monomers of the dimer have the same molecular weight of 45 kDa.  

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Related Questions

What is a monogenic trait?
What is a polygenic trait?
What is epistasis?
The traits studied by Mendel are examples of

Answers

Monogenic trait refers to a trait that is determined by a single gene, such as hair or eye color. Polygenic trait refers to a trait that is influenced by multiple genes, such as height or skin color.

Epistasis refers to the interaction between different genes, where the expression of one gene affects the expression of another gene.

The traits studied by Mendel are examples of monogenic traits. This is because Mendel conducted his experiments on pea plants, which have traits that are determined by a single gene pair. His work helped to establish the principles of inheritance and the idea that traits are passed down from parents to offspring in a predictable manner.In conclusion, monogenic traits are determined by a single gene, while polygenic traits are influenced by multiple genes.

Epistasis refers to the interaction between different genes. The traits studied by Mendel are examples of monogenic traits.

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Identify how the steps in the S.H.A.D.E process are illustrated in the following case: Dianne, a 30-year-old part-time receptionist, has been experiencing chest pains in the center of her chest for more than 2 months. The discomfort lasts from 5 to 10 minutes. These pains seem to occur after dinner if she lies down but not with exercise or at work. Dianne is concerned about whether these are due to her heart because her father died of a heart attack at age 62 . She also worries because she is a bit overweight, being 5 4 −1 and 170 pounds, and does not exercise regularly. After asking about the exact location, timing. and frequency of her symptoms and determining that Diame has not been a cigarette smoker, her doctor did a physical examination. The doctor examined Dianne's heart, stomach, and lungs; took her blood pressure; and did not find any abnormalities. Finally, the doctor checked Dianne's previous records and found that her LDL, or bad cholesterol level, was within the normal range. Because of the concern about her heart, Dianne was sent for an exercise stress test in which she was hooked up to an electrocardiogram and ran in place on a treadmill. The doctor asked Dianne to report if she experienced any chest pain. Before, during, and after the examination, her blood pressure was taken. Noting that her stress test and examination were negative for any abnormalities, the doctor asked Dianne to help him by taking liquid antacid immediately whenever the pain occurs, recording what happens and how long it takes. Dianne found that on three occasions, within 30 seconds of taking the liquid antacid, the pain disappeared. After telling her doctor this information, the doctor confidently said: "There is no evidence of heart disease. Your symptoms are caused by acid reflux or heartburn."

Answers

The steps in the S.H.A.D.E process that are illustrated in the given case are S: Symptom Analysis: In this step, Dianne, a 30-year-old part-time receptionist, has been experiencing chest pains in the centre of her chest for more than 2 months. The discomfort lasts from 5 to 10 minutes.

She informed the doctor that these pains seem to occur after dinner if she lies down but not with exercise or at work. The doctor, after knowing the location, timing, and frequency of her symptoms, asked more questions to know about her health history and if she was a cigarette smoker.

H: History Analysis: Dianne is concerned about whether these are due to her heart because her father died of a heart attack at age 62. She also worries because she is a bit overweight, being 5 4 −1 and 170 pounds, and does not exercise regularly. The doctor checked her previous records and found that her LDL, or bad cholesterol level, was within the normal range.

A: Assessment and Action: The doctor did a physical examination to examine Dianne's heart, stomach, and lungs; took her blood pressure; and did not find any abnormalities. Because of the concern about her heart, Dianne was sent for an exercise stress test in which she was hooked up to an electrocardiogram and ran in place on a treadmill.

Before, during, and after the examination, her blood pressure was taken. Noting that her stress test and examination were negative for any abnormalities, the doctor asked Dianne to help him by taking liquid antacids immediately whenever the pain occurs, recording what happens and how long it takes.

D: Decision and Diagnosis: Dianne found that on three occasions, within 30 seconds of taking the liquid antacid, the pain disappeared. After telling her doctor this information, the doctor confidently said: "There is no evidence of heart disease. Your symptoms are caused by acid reflux or heartburn."

E: Evaluation and Education: The doctor educated Dianne on the symptoms and what actions to take.

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All Of The Following Are Products Of Glycolysis EXCEPT: O NADH O Coenzyme A O Pyruvate O2 ATP MoleculesAll Of The Following Are Products Of Glycolysis EXCEPT: O NADH O Coenzyme A O Pyruvate O2 ATP Molecules

Answers

Answer:

All of the following are products of glycolysis EXCEPT: O2 (oxygen).

Glycolysis is the initial step in cellular respiration and occurs in the cytoplasm of cells. It involves the breakdown of glucose to produce energy and various metabolic products. The products of glycolysis include NADH, pyruvate, and ATP molecules. NADH is an energy carrier molecule, pyruvate is a three-carbon compound, and ATP is the primary energy currency of cells. These three are the products of glycolysis. However, oxygen (O2) is not directly produced by glycolysis. Instead, oxygen plays a crucial role in subsequent steps of cellular respiration, such as the citric acid cycle and oxidative phosphorylation, where it acts as the final electron acceptor.

To summarize, the products of glycolysis include NADH, pyruvate, and ATP molecules, but oxygen (O2) is not a direct product of glycolysis.

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the muscularis mucosae function in ways quite similar to which other anatomical feature of the human body?

Answers

The muscularis mucosae function in ways quite similar to the muscle fibers of the arteriole walls in the human body.

The muscularis mucosae is a thin layer of muscle found in the tunica mucosa of hollow organs of the human body, such as the gastrointestinal tract. The muscularis mucosae is a type of smooth muscle, and it is located beneath the mucous membrane's epithelium.The muscularis mucosae has two principal layers of smooth muscle cells, which are orientated in an internal circular and an external longitudinal direction. These two layers' orientation gives the mucous membrane the ability to undulate, which propels food through the digestive system.

Aside from being present in the human body, the muscle fibers of arteriole walls are a structural feature of the cardiovascular system that controls blood flow through the arteries by contracting and relaxing. The contraction of the arteriole smooth muscle layer reduces blood flow to a region, while the relaxation of the smooth muscle layer increases blood flow to a region.

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Match the directions to the statements. The scapula (shoulder blade) is The elbow is The nose is The ankle is The brain is spinal cord. The breasts are the chest muscles. The muscles are the to skin.

Answers

The scapula is posterior. The elbow is anterior. The nose is superior . The ankle is distal. The brain is superior to the spinal cord. The breasts are anterior to the chest muscles. The muscles are deep to the skin.

The scapula (shoulder blade) is located on the posterior (back) side of the body. It forms part of the shoulder girdle and provides attachment sites for muscles involved in arm movement.

The elbow joint is located on the anterior (front) side of the arm. It allows flexion and extension movements and is formed by the articulation between the humerus, radius, and ulna bones.

The nose is situated superior (above) to other facial features. It is the central organ of the respiratory system and plays a role in smelling and breathing.

The ankle joint is found distal (away from the body) to the leg. It connects the lower leg bones (tibia and fibula) with the foot bones and allows for movements such as dorsiflexion and plantarflexion.

The brain is superior (above) to the spinal cord. It is the command center of the central nervous system, responsible for coordinating and controlling body functions, cognition, and sensory perception.

The breasts are positioned anterior (in front) to the chest muscles. They are glandular organs in females that produce milk for breastfeeding.

The muscles are deep to the skin. Muscles are the contractile tissues responsible for body movement and are located beneath the skin and subcutaneous tissue. They provide support, stability, and movement to the body.

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What type of membrane transporter moves two species in the same direction across a membrane?
a. Antiporter
b. None of the other answers are correct
c. Symporter
d. Cotransporter
e. Uniporter Which of the following is not true of chymotrypsin?
a. It contains a superoxide hole
b. It is a serine protease
c. It acts on the carboxyl side of bulky amino acids
d. It contains an S1 pocket
e. It is synthesized as an inactive zymogen

Answers

The type of membrane transporter that moves two species in the same direction across a membrane is called a symporter.

Symporters are membrane proteins that transport two different molecules or ions across the membrane in the same direction. These transporters utilize the energy derived from the electrochemical gradient of one of the transported species to drive the co-transport of the other species. By coupling the movement of two substances, symporters facilitate the uptake or secretion of specific molecules across cell membranes, allowing for various physiological processes and cellular functions.

Regarding chymotrypsin, the statement that is not true is: "It contains a superoxide hole." Chymotrypsin is a serine protease, which means it belongs to a class of enzymes that catalyze the hydrolysis of peptide bonds in proteins. It specifically acts on the carboxyl side of bulky amino acids. Chymotrypsin contains an S1 pocket, which is a specific binding site that accommodates large hydrophobic amino acid residues. Additionally, chymotrypsin is synthesized as an inactive zymogen, called chymotrypsinogen, which is then activated by proteolytic cleavage to form the active enzyme chymotrypsin.

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Which of these sputum strains would one use to test for a viral respiratory culture?
A. Gram stain
B. Direct fluorescent antibody stain
C. Wright-Giemsa stain
D. Acid-fast stain

Answers

The correct option to use for the test of a viral respiratory culture is the Direct fluorescent antibody stain.

Viral respiratory culture testing is a lab test that identifies a respiratory virus that can cause respiratory tract infections. Infections of the upper respiratory tract, including the nose, throat, and sinuses, are common. The lower respiratory tract, which includes the lungs and airways, can also be affected by respiratory viruses.

Respiratory specimens include nasopharyngeal and oropharyngeal swabs, sputum, bronchoalveolar lavage, and tracheal aspirates. The type of specimen collected for viral culture is determined by the type of virus suspected or the clinical presentation of the patient.When it comes to the identification of respiratory viruses, viral cultures are now seldom used in clinical practice due to their low sensitivity, lengthy turnaround times, and difficulties maintaining the viability of the viruses during transport.

Other techniques like RT-PCR, Luminex xTAG Respiratory Viral Panel, and FilmArray Respiratory Panel are now used to diagnose respiratory viruses.

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which depends on the skeletal muscle pump and changes during breathing

Answers

Answer:

veins

Explanation:

vein

As muscle contract and release, the blood is milked through the veins towards the heart pressure changes that occur in the thorax during breathing also aid blood return.

The complete oxidation of one molecule of the fatty acid, CH 3(CH2) 10COOH, produces how many molecules of ATP 35 25 95 97 72

Answers

The complete oxidation of one molecule of the fatty acid, CH3(CH2)10COOH, produces 97 molecules of ATP.

The process of fatty acid oxidation occurs in multiple steps and involves the breakdown of fatty acids to produce ATP. The fatty acid CH3(CH2)10COOH is a fatty acid with 12 carbon atoms.

During fatty acid oxidation, the fatty acid is broken down into two-carbon units through a series of reactions called beta-oxidation. Each two-carbon unit goes through several enzymatic reactions, including oxidation, hydration, and oxidation, resulting in the production of acetyl-CoA.

For each round of beta-oxidation, one molecule of FADH2 and one molecule of NADH are generated, which are then used in the electron transport chain to produce ATP. Each round of beta-oxidation generates 5 molecules of ATP.

Since the fatty acid CH3(CH2)10COOH has 6 two-carbon units, the complete oxidation of one molecule of this fatty acid will produce 6 x 5 = 30 molecules of ATP through beta-oxidation. Additionally, the oxidation of the resulting acetyl-CoA molecules in the citric acid cycle can generate an additional 12 molecules of ATP. Therefore, the complete oxidation of one molecule of this fatty acid yields a total of 30 + 12 = 42 molecules of ATP.

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1. List one piece of evidence that indicates eukaryotes arose from prokaryotes and explain why this evidence supports the endosymbiotic hypothesis. 2. Why is it difficult to determine what LUCA was and what the relationships amono Bacteria. Archaea. and Eukarva are using gene trees?

Answers

Presence of organelles (e.g., mitochondria, chloroplasts) in eukaryotic cells suggests they arose from prokaryotes. Organelles share similarities with bacteria and cyanobacteria, indicating an endosymbiotic hypothesis where prokaryotes were engulfed by ancestral eukaryotic cells.

Challenges in determining LUCA and relationships: Gene trees face difficulties in determining LUCA and relationships among Bacteria, Archaea, and Eukarya due to horizontal gene transfer (HGT) prevalent in bacteria and archaea. Scarce evidence from LUCA's time period and complexity of relationships further hinder precise classification based on genetic sequences. Shared features and lateral gene transfer add complexity, making it challenging to construct definitive gene trees for these domains.

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Why are tropical rainforest soils so infertile?

Answers

Tropical rainforest soils are often characterized by their relatively low fertility compared to other types of soils.

There are several factors that contribute to their infertile nature. One of the primary reasons is rapid nutrient cycling. The warm and moist conditions in tropical rainforests promote the speedy decomposition of organic matter. As a result, nutrients are quickly absorbed and recycled by vegetation, leaving little time for their accumulation in the soil.

The nutrients are efficiently taken up by the dense plant life, leaving limited amounts available for other organisms. Additionally, leaching is a significant factor. The heavy rainfall in tropical rainforests leads to leaching, where nutrients are washed away from the topsoil. This process carries away essential nutrients, such as nitrogen, potassium, and phosphorus, which are vital for plant growth.

Consequently, the leached soils become deficient in these nutrients, rendering them less fertile. Furthermore, the high temperatures and humidity accelerate chemical weathering processes. Rainwater reacts with minerals in the soil, leading to their breakdown and further depletion of nutrients.

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1. What is chemiosmotic coupling and how is it used by cells to produce ATP? 2. What is redox potential and how is it used in electron transport chains? 3. What two ways do plant cells use to synthesize ATP?

Answers

Chemiosmotic coupling is a process by which cells utilize an electrochemical gradient to produce adenosine triphosphate (ATP). It involves the coupling of electron transport and ATP synthesis, where the movement of protons across a membrane drives the synthesis of ATP. The key player in this process is ATP synthase, a membrane-bound enzyme that acts as a molecular turbine.

During electron transport, electrons are transferred along a series of electron carriers embedded in the mitochondrial inner membrane (in the case of eukaryotic cells) or the plasma membrane (in the case of prokaryotic cells). As electrons move through the electron transport chain, protons are pumped across the membrane, establishing a proton gradient. This proton gradient creates a difference in proton concentration and charge across the membrane.

Redox potential, also known as reduction potential, is a measure of the tendency of a molecule to gain or lose electrons. In electron transport chains, redox potential is used to drive the flow of electrons from molecules with lower redox potential to molecules with higher redox potential.

In an electron transport chain, electrons are transferred from electron donors (such as NADH or FADH2) to electron acceptors (such as oxygen or other terminal electron acceptors). Each electron carrier in the chain has a specific redox potential, which determines its ability to accept or donate electrons.

Plant cells use two main pathways to synthesize ATP: oxidative phosphorylation and photophosphorylation. Oxidative phosphorylation occurs in the mitochondria and involves the transfer of electrons through the electron transport chain. During this process, energy is released as electrons flow from electron donors, such as NADH, to the terminal electron acceptor, which is typically oxygen. The energy released is used to generate a proton gradient across the mitochondrial inner membrane, which is then used by ATP synthase to produce ATP.

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which of the following factors does not contribute to muscle fatigue associated with prolonged exercise?

Answers

The factor that does not contribute to muscle fatigue associated with prolonged exercise is not explicitly mentioned in the question.

Muscle fatigue associated with prolonged exercise is a complex phenomenon influenced by various factors.

These factors can include metabolic changes, depletion of energy stores, accumulation of metabolic byproducts, impaired muscle contractility, and central nervous system fatigue.

However, the question does not specify which factor does not contribute to muscle fatigue.

To identify the factor that does not contribute to muscle fatigue, it would be necessary to consider factors that are known to play a role in muscle fatigue and exclude those that do not have a significant impact.

Some potential factors that might not contribute to muscle fatigue include psychological factors (e.g., motivation, attention), muscle fiber type distribution (as certain fiber types may be more resistant to fatigue), and genetic factors (individual variations in fatigue resistance).

However, without further information, it is not possible to determine the specific factor that does not contribute to muscle fatigue associated with prolonged exercise.

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niacin can be made in the body from the amino acid tryptophan. a. true b. false

Answers

Niacin can be made in the body from the amino acid tryptophan is true. Here's an explanation of the statement:Niacin is a water-soluble vitamin and an essential nutrient that is crucial for human health.

The body needs niacin for several metabolic processes, including the production of energy. Niacin also plays a role in DNA repair, cell signaling, and the regulation of cholesterol levels.Niacin can be obtained from the diet, particularly from animal-based foods such as meat, fish, and poultry, as well as from plant-based sources like nuts, legumes, and grains.

However, niacin can also be synthesized in the body from the essential amino acid tryptophan. Tryptophan is an amino acid found in many dietary sources, including meat, fish, dairy, and some plant-based foods.

The process of synthesizing niacin from tryptophan is known as the kynurenine pathway.In summary, niacin can be made in the body from the amino acid tryptophan, so it is true.

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biofilms provide an alternative means for bacteria to attach to surfaces within the body. true false

Answers

The statement is True. Biofilms serve as an alternative method for bacteria to adhere to surfaces within the body.

Biofilms are complex communities of microorganisms, primarily bacteria, that form a protective matrix on surfaces such as tissues or medical implants. In a biofilm, bacteria aggregate and adhere to each other and the surface, forming a slimy and cohesive structure. This matrix provides stability and protection, shielding the bacteria from the immune system and antibiotics.

Biofilms can form on various surfaces within the body, including tissues, organs, and medical devices like catheters or prosthetic implants. By attaching to surfaces through biofilm formation, bacteria can persist and thrive, leading to chronic infections and increased resistance to treatment.

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In a nondividing cell, the flow of membrane between cellular compartments is balanced, with the inward flow of membrane (endocytosis) matching the outward flow (exocytosis). Why is it important to maintain this balance? Would you expect the same balanced flow in actively dividing gut epithelial cells? Explain your answer.

Answers

Maintaining a balanced flow is important to ensure proper cell function and maintain cellular homeostasis.The balance between epithelial cells may be altered.

In a nondividing cell, endocytosis brings in new membrane components, such as receptors and nutrients, while exocytosis removes waste products and exports molecules. This balance helps maintain the integrity and functionality of cellular compartments and facilitates communication with the extracellular environment.

In actively dividing gut epithelial cells, the balance between endocytosis and exocytosis may be altered. During cell division, there is a need for increased membrane synthesis and expansion to accommodate the growth and division of the cell. The cell may undergo extensive membrane remodeling, including the formation of the mitotic spindle and the partitioning of organelles. As a result, there may be a higher rate of exocytosis to supply new membrane material and maintain membrane integrity during cell division. This increased membrane turnover is essential for cell division and the proper formation of daughter cells.

While the balance between endocytosis and exocytosis is crucial in nondividing cells, actively dividing gut epithelial cells may experience an altered membrane flow with a higher emphasis on exocytosis to support cell division and growth.The equilibrium between endocytosis and exocytosis allows for the regulation of membrane composition, surface area, and the trafficking of molecules in and out of the cell.

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The Ames Test uses a Salmonella enterica mutant strain that is unable to grow in the absence of histidine. How is the mutant strain used to test whether a compound is mutagenic?
A. The strain is used to measure rat liver enzymatic activity.
B. The strain is used to estimate how many forward mutations a tested compound causes that lead to the mutant phenotype.
C. The strain is used to determine how many more back mutations a tested compound causes that restore wild-type growth.
D. The strain is used produce the histidine needed for the test.
E. The strain is used for DNA sequencing to determine the number of mutations caused by a tested compound.

Answers

The Ames Test uses a Salmonella enterica mutant strain that is unable to grow in the absence of histidine. The mutant strain is used to test whether a compound is mutagenic. The answer is option B.

The strain is used to estimate how many forward mutations a tested compound causes that lead to the mutant phenotype. The Ames Test is a method of detecting mutagens. It is a biological assay that uses bacteria to test the mutagenicity of chemical compounds. The test is named after its inventor, Bruce Ames, who developed it in the 1970s. The Ames Test is widely used in the field of toxicology to assess the potential health risks posed by new chemicals and drugs.

The test is designed to identify mutagenic chemicals, which are chemicals that cause genetic mutations in living cells. The Ames Test is based on the use of bacterial strains that are unable to synthesize histidine due to a mutation in the his gene. These bacteria can only grow in the presence of histidine. The test uses a mutagenic compound to induce mutations in the bacterial DNA. If a chemical compound is mutagenic, it will cause mutations in the bacterial DNA that restore the ability of the bacteria to grow in the absence of histidine.

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what cell structure provides an interconnected meshwork that gives animal cells tensile strength and support?

Answers

The cell structure that provides an interconnected meshwork giving animal cells tensile strength and support is the cytoskeleton.

The cytoskeleton is a network of protein filaments that spans the cytoplasm of animal cells. It plays a crucial role in maintaining cell shape, providing mechanical support, and facilitating cellular movement. The three main components of the cytoskeleton are microfilaments, intermediate filaments, and microtubules.

Microfilaments, composed of actin proteins, are the thinnest filaments of the cytoskeleton. They form a dense web-like structure just beneath the cell membrane, contributing to cell shape and providing mechanical support. Microfilaments also participate in cellular processes such as cell division and cell migration.

Intermediate filaments are slightly thicker and more rigid than microfilaments. They provide structural support to cells by forming a sturdy scaffold inside the cytoplasm. Intermediate filaments are made up of various proteins, including keratins, lamins, and vimentins, which give cells tensile strength and resistance to mechanical stress.

Microtubules are the largest filaments of the cytoskeleton and are composed of tubulin proteins. They form hollow tubes that radiate throughout the cell and play a crucial role in cell division, intracellular transport, and maintaining cell shape. Microtubules also provide structural support to animal cells, acting as tracks for motor proteins to transport vesicles and organelles within the cell.

In summary, the cytoskeleton is the cell structure that provides an interconnected meshwork, consisting of microfilaments, intermediate filaments, and microtubules, which give animal cells tensile strength and support. It is essential for maintaining cell shape, facilitating cellular movement, and ensuring structural integrity.

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In a population 80% of the individuals are homozygous dominant, and 16% are heterozygous, and 4% are homozygous recessive- the frequency of the recessive allele Q is?
01 or 10%
6 or 60%
2 or 20%
5 or 50%
If the analysis shows that p2 25 and 2pq.50 and q2+ 25, the homozygous dominant genotype frequency is
10
20
25
30
If the genotypre frequency in a population is 16+48+.36 then the frequency of the recessive allele
6
8
f the genotypre frequency in a population is .16+ 48+36 then the frequency of the dominate allele
is
A
.6
8

Answers

The frequency of the recessive allele is 20%, the frequency of homozygous dominant genotype is 25%, the frequency of the recessive allele is 60%, and the frequency of the dominant allele is 60%.

To find the frequency of the recessive allele Q, the values of the frequency of homozygous dominant, heterozygous, and homozygous recessive need to be known, which are 80%, 16%, and 4%, respectively. After calculating, the frequency of the recessive allele Q was found to be 20%. Next, to determine the homozygous dominant genotype frequency, the formula p2 + 2pq + q2 = 1 was used, and by substituting the values, the frequency of the homozygous dominant genotype was found to be 25%.

In the next question, the genotype frequency in the population is given as 16+48+.36, from which the frequency of the recessive allele was determined to be 60%.In the last question, the genotype frequency in the population is given as 16+48+.36, from which the frequency of the dominant allele was determined to be 60%.

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1) Select two conditions relevant to the Hardy-Weinberg equilibrium. Explain using specific examples how a change in each of those conditions can alter the conditions such that there will be selective pressure to drive evolutionary change. 2) Explain using a specific example how frequency-dependent selection can act as a negative selective pressure.

Answers

1) Two conditions relevant to the Hardy-Weinberg equilibrium are genetic drift and natural selection

2) example is the frequency-dependent selection observed in predator-prey interactions

1) Two conditions relevant to the Hardy-Weinberg equilibrium are genetic drift and natural selection. Genetic drift refers to the random fluctuations in allele frequencies within a population due to chance events. For example, in a small population, genetic drift can have a significant impact. If, by chance, a particular allele becomes overrepresented or underrepresented, it can lead to a change in the gene pool and subsequent evolution. Natural selection, on the other hand, acts on heritable traits that confer a fitness advantage or disadvantage. For instance, consider a population of insects with different color morphs living on different colored flowers. If a change in environmental conditions favors one color morph over others (e.g., predators can detect one color more easily), natural selection will drive the frequency of that advantageous morph to increase, leading to evolutionary change.

2)Frequency-dependent selection occurs when the fitness of a particular phenotype depends on its frequency within a population. One example is the frequency-dependent selection observed in predator-prey interactions. Let's consider a hypothetical scenario involving a population of fish and their primary predator, a bird species. If the fish population consists of both red and blue individuals, and the bird has a higher preference for blue fish, the frequency of blue fish will decrease over time due to predation. However, as the frequency of blue fish decreases, the bird's preference may shift towards the more abundant red fish, resulting in negative selection pressure for the red fish. In this case, the fitness of each phenotype depends on its frequency relative to the other, leading to a cyclic fluctuation in phenotype frequencies within the population.

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mRNA is the result of O (C) Translation (B) Transcription O (A) Replication O (D) A-C are incorrect

Answers

mRNA is the result of (B) Transcription. Transcription is the process in which an RNA molecule is synthesized from a DNA template.

During transcription, the DNA strand serves as a template for the synthesis of a complementary RNA molecule, which is known as messenger RNA (mRNA). The mRNA carries the genetic information from the DNA to the ribosomes in the cytoplasm, where it serves as a template for protein synthesis during the process of translation. Replication, on the other hand, is the process of copying DNA to produce identical DNA molecules. It occurs during cell division to ensure that each daughter cell receives a complete set of genetic information.

Translation is the process in which the genetic information carried by mRNA is used to synthesize proteins. It takes place on the ribosomes and involves the interaction of mRNA with transfer RNA (tRNA) molecules and the assembly of amino acids into a polypeptide chain. Therefore, mRNA is produced through the process of transcription, where the DNA sequence is transcribed into a complementary RNA sequence.

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which of the following children is heterozygous for a given trait?
Indicate as many as are relevant: AA, aa, Aa, Aa

Answers

The correct option is c. The child that is heterozygous for a given trait is Aa.

In genetics, an individual can inherit two alleles for a particular trait, one from each parent. These alleles can be either dominant or recessive. Heterozygous refers to a genotype where the individual has two different alleles for a specific trait. In the options, AA represents a homozygous dominant genotype, meaning both alleles are the same and dominant. The aa genotype represents a homozygous recessive genotype, where both alleles are the same and recessive.

The Aa genotype, on the other hand, represents a heterozygous genotype. In this case, the individual has inherited one dominant allele (A) and one recessive allele (a). Heterozygosity often results in the dominant allele being expressed phenotypically, masking the presence of the recessive allele.

Therefore, among the given options, the child who is heterozygous for a given trait is Aa.

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of a mitochondrion in producing ATP.
(3)
(b)
A scientist investigated ATP production in a preparation of isolated mitochondria. He suspended the mitochondria in an isotonic solution and added a suitable respiratory substrate together with ADP and phosphate. He bubbled oxygen through the preparation.
(1) Why was the solution in which the mitochondria were suspended isotonic?
(ii)
(1)
Explain why the scientist did not use glucose as the respiratory substrate.
(2)
(ii) Explain why the oxygen concentration would
change during this investigation.

Answers

(a) Mitochondrial respiration produces ATP. All eukaryotic cells have double-membrane mitochondria. Cellular respiration releases ATP by oxidizing glucose.

Mitochondrial oxidative phosphorylation generates ATP. Mitochondrial inner membranes generate ATP. Internal membrane cristae boost electron transport chain surface area. Inner membrane electron transport chains generate ATP.

NADH and FADH2 electron acceptors pump protons across the inner membrane. The intermembrane gap proton gradient increases ATP synthase synthesis. Electron transport chain proton gradients generate ATP.

(b) Mitochondrial isotonic solution balanced water. Isotonic solutions balance mitochondrial water—Mitochondria benefit. No water movement occurs in isotonic solutions.

Mitochondrial membranes block glucose. It becomes acetyl-CoA and pyruvate outside mitochondria. Thus, researchers used ATP-producing respiratory substrates.

This experiment would change oxygen concentration since the electron transport cycle consumes oxygen. Electron transport converts oxygen to water. Electron transfer lowers oxygen concentration. As oxygen is used, cellular respiration releases CO2 from respiratory substrates, increasing CO2 concentration.

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How does activation of glycogen synthase reduce blood glucose levels? Be specific in explaining what is happening to glucose on a molecular level. -Akt stimulation also down regulates the enzyme FOXO1. Explain how this reduces blood glucose levels. Again, be specific about what is happening to glucose on a
molecular level.

Answers

Activation of glycogen synthase and downregulation of the enzyme FOXO1 are two mechanisms that contribute to the reduction of blood t's examine each process in detail: glucose levels.

Activation of Glycogen Synthase:

Glycogen synthase is an enzyme responsible for the synthesis of glycogen, a storage form of glucose in the liver and muscles. When glycogen synthase is activated, it promotes the conversion of excess glucose into glycogen, thus reducing the amount of glucose present in the bloodstream.

Molecular Level Explanation:

When blood glucose levels are high, insulin is released by the pancreas in response to elevated blood sugar. Insulin triggers a signaling cascade that leads to the activation of the enzyme glycogen synthase. Activated glycogen synthase plays a crucial role in glycogenesis, the process of glycogen synthesis.

At a molecular level, glycogen synthase is phosphorylated and inactivated under normal conditions. However, insulin signaling activates a protein kinase called Akt (also known as protein kinase B), which phosphorylates and inhibits glycogen synthase kinase 3 (GSK-3). Inhibition of GSK-3 prevents it from phosphorylating and inhibiting glycogen synthase.

Therefore, when GSK-3 is inhibited, glycogen synthase becomes dephosphorylated and active. Active glycogen synthase catalyzes the addition of glucose molecules to the growing glycogen chain, effectively storing excess glucose as glycogen in the liver and muscles. This process helps to lower blood glucose levels by reducing the available glucose in the bloodstream.

Downregulation of FOXO1:

FOXO1 (Forkhead box protein O1) is a transcription factor involved in regulating the expression of genes related to glucose metabolism, including gluconeogenesis, the process by which the liver produces glucose. Akt stimulation, which occurs downstream of insulin signaling, leads to the downregulation of FOXO1.

Molecular Level Explanation:

Akt phosphorylates FOXO1, promoting its exclusion from the cell nucleus and preventing it from binding to the DNA and activating genes involved in gluconeogenesis. As a result, the expression of enzymes involved in glucose production, such as phosphoenolpyruvate carboxykinase (PEPCK) and glucose-6-phosphatase (G6Pase), is reduced.

By downregulating FOXO1 and inhibiting gluconeogenesis, the liver's glucose production is decreased, further contributing to the reduction of blood glucose levels.

In summary, the activation of glycogen synthase promotes glycogen synthesis, storing excess glucose as glycogen, while downregulation of FOXO1 inhibits gluconeogenesis, reducing the production of new glucose. Both processes work together to lower blood glucose levels by regulating glucose utilization and production in the body.

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Which Of The Following Statements Is Not True Regarding Varicella And Herpes Zoster? Varicella Is The Manifestation Of The Initial Infection, Whereas Herpes Zoster Is The Recurrent Form Of The Latent Disease. Both Diseases Are Caused By Human Herpes Virus-3 (HHV-3). Varicella Is A Disease Found Primarily In Children, Whereas Herpes Zoster Is Found Primarily

Answers

Varicella is the manifestation of the initial infection, whereas herpes zoster is the recurrent form of the latent disease. Both diseases are caused by Human Herpes Virus-3 (HHV-3).

Varicella, commonly known as chickenpox, is indeed the initial infection caused by the varicella-zoster virus (HHV-3). It primarily affects children and is characterized by a generalized rash, fever, and flu-like symptoms. Once a person has had varicella, the virus remains in a dormant state in the body's nerve cells.

Herpes zoster, commonly known as shingles, is the reactivation of the dormant varicella-zoster virus in individuals who have previously had varicella. It can occur at any age, including in children, but it is more commonly seen in adults. Herpes zoster is characterized by a painful rash that typically occurs in a band or strip on one side of the body.

Both varicella and herpes zoster are caused by the same virus, HHV-3, but they represent different manifestations of the infection. Varicella is the primary infection, while herpes zoster is the reactivation of the virus. It is important to note that both varicella and herpes zoster can occur in individuals of any age, although their frequency may vary.

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Which Of The Following Statements Is TRUE Regarding The Free Energy Diagrams Below A. The Free Energy Change Of Reaction 8 Is Greater Than That Of Reaction A. B. Reaction 8 Is Faster Than Reaction A. C Both Statements Are True. The Difference In Free Energy Between The Substrate And Product Of A Reaction Catalyzed By Enzyme A Is Negative And Small.

Answers

The correct answer is: C. Both statements are true.In the context of the given information, both statements are accurate.

The free energy change of reaction 8 is greater than that of reaction A, indicating a larger energy difference between the reactants and products in reaction 8 compared to reaction A. This suggests that reaction 8 is thermodynamically less favorable than reaction A.Additionally, if reaction 8 has a higher free energy barrier, it implies that reaction 8 is slower than reaction A.

The higher energy barrier means that more energy is required to initiate the reaction, leading to a slower rate of reaction compared to reaction A. Furthermore, the statement about the difference in free energy between the substrate and product of a reaction catalyzed by enzyme A being negative and small is not provided in the given options. Therefore, it cannot be determined based on the information provided.

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The lac operon consists of regulatory regions such as the promoter as well as the structural genes lacZ, lacy, and lacA, which code for proteins involved in lactose metabolism. The operon model describes expression in prokaryotes. Describe this model and the essential difference in the way in which expression is regulated in eukaryotes. What would be the outcome of a missense mutation in one of the structural genes of the lac operon? Why? Explain

Answers

The operon model in prokaryotes, such as the lac operon, regulates gene expression based on the presence or absence of inducers like lactose. In eukaryotes, gene expression is regulated through more complex mechanisms involving transcription factors, enhancers, and chromatin modifications.

The operon model is a regulatory mechanism found in prokaryotes, such as bacteria, that controls gene expression. In the lac operon, the regulatory regions include the promoter, which initiates transcription, and the operator, which controls the access of RNA polymerase to the structural genes. The structural genes lacZ, lacY, and lacA encode proteins involved in lactose metabolism.

In prokaryotes, the lac operon is regulated by the presence or absence of lactose and the presence of a regulatory protein called the lac repressor. When lactose is present, it binds to the lac repressor, causing a conformational change that allows RNA polymerase to bind to the promoter and initiate transcription of the structural genes. This is known as inducible regulation.

In contrast, gene expression in eukaryotes is more complex and involves additional regulatory mechanisms such as transcription factors, enhancers, and chromatin modifications. Eukaryotic gene expression is tightly regulated and can be influenced by a variety of internal and external factors.

A missense mutation in one of the structural genes of the lac operon would result in a change in the amino acid sequence of the encoded protein. The outcome of this mutation would depend on the specific amino acid change and its effect on protein structure and function. It could potentially lead to a loss of enzymatic activity or altered protein function, which may affect lactose metabolism in the cell.

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DNA methylation and histone acetylation are both examples of:
Group of answer choices
translocation
genetic mutation
epigenetic changes to chromosomes
karyotypes
chromosomal rearrangements

Answers

DNA methylation and histone acetylation are both examples of epigenetic changes to chromosomes.

DNA methylation and histone acetylation are both epigenetic modifications that play crucial roles in gene regulation and chromatin structure. DNA methylation involves the addition of a methyl group to DNA molecules, typically at cytosine residues, which can lead to gene silencing and transcriptional repression. Histone acetylation, on the other hand, involves the addition of acetyl groups to histone proteins, resulting in relaxed chromatin structure and increased gene expression. These epigenetic modifications are reversible and can be influenced by various environmental factors, influencing gene activity without altering the underlying DNA sequence. They are essential for developmental processes, cellular differentiation, and maintaining cellular identity.

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If a cell containing 25% salt is placed in a glass of water with 10% salt, the cell is_compared to the surrounding water.
Select one:
a. hypotonic
b. isotonic
c. hypertonic
d. None of the answers are correct.
Clear my choice

Answers

The correct answer is (c) hypertonic. When a cell containing 25% salt is placed in a glass of water with 10% salt, the cell is hypertonic compared to the surrounding water.

The terms hypotonic, isotonic, and hypertonic describe the relative concentrations of solutes in a solution compared to a reference solution. In this scenario, the cell contains 25% salt, while the surrounding water has a lower concentration of salt at 10%. When a solution is hypertonic, it means that it has a higher concentration of solutes compared to the reference solution. In this case, the cell's salt concentration of 25% is higher than the 10% salt concentration in the surrounding water. As a result, water molecules will move out of the cell, following the concentration gradient, to equalize the concentrations. This can lead to cell shrinkage or dehydration. Therefore, the cell is hypertonic compared to the surrounding water.

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According to the sequence below (note that only one strand of double stranded DN written in the 5'3' direction, and the gene of interest is underlined), (A) design two primers (5 oligonucleotides in length) that could be used in PCR to amplify the template strand and its complement (indicate 5' and 3' end). (B) List other ingredients you need in PCR besides the primers. (6 pts)

Answers

To amplify the template strand and its complement in PCR, two primers of 5 oligonucleotides in length need to be designed additional ingredients required for PCR include a DNA template, DNA polymerase enzyme, nucleotides (dNTPs), buffer solution, and magnesium ions.

(A) To design the primers for PCR amplification, the 5' and 3' ends need to be indicated. Considering the given sequence with the gene of interest underlined, the primers should be designed in a way that they complement the template strand and its complement. The 5' end of a primer corresponds to the starting point, while the 3' end indicates the ending point of the primer sequence.

(B) In addition to the primers, several other ingredients are required for PCR. These include a DNA template, which serves as the starting material for amplification, and a DNA polymerase enzyme, such as Taq polymerase, that catalyzes the synthesis of new DNA strands. Nucleotides, also known as dNTPs (deoxynucleotide triphosphates), are the building blocks for DNA synthesis.

PCR also requires a buffer solution to maintain optimal pH and ionic conditions for enzymatic reactions to occur. Magnesium ions (Mg2+) are often included in the PCR reaction mix as they are essential for the proper function of DNA polymerase.

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