How to determine the enzymes used to cut plasmid in cloning.

Answers

Answer 1
When cloning by restriction digest and ligation, you use restriction enzymes to cut open a plasmid (backbone) and insert a linear fragment of DNA (insert) that has been cut by compatible restriction enzymes. An enzyme, DNA ligase, then covalently binds the plasmid to the new fragment thereby generating a complete, circular plasmid that can be easily maintained in a variety of biological systems. Read on for an in-depth breakdown of how to do perform restriction digests.

Related Questions

aquatic plant emits bubbles of oxygen when placed under a bright light. The table shows the results of an experiment to show the effect of light intensity on bubble production. Use the data in the table to answer the question.


Oxygen Production
Distance From Light (cm)
Bubbles Produced per Minute
10
39
20
22
30
8
40
5


Distance From Light (cm)
10
20
30
40


Oxygen Production
Bubbles Produced per Minute
39
22
8
5



What conclusion about photosynthesis is supported by the evidence from this experiment? Explain your reasoning.

Answers

more light means more photosynthesis means more oxygen

closer to light the more oxygen is produced

distance from light & oxygen produced is in an inverse relationship which means when one goes up the other goes down

According to the data on the table, when the distance from the light increased, the bubbles produced per minute have decreased. The relationship between the two variables shows an inverse one. This means that if one variable increases, then the other one decreases, and vice-versa. Using this information, we can conclude that when the aquatic plant is placed far from light, it gets less sun exposure. This affects the rate of photosynthesis, including its by-products. When the rate of photosynthesis is slow, the oxygen produced as a waste product is also low.

quizlet

. Taiga forests are _____
and receive_______
rainfall than temperate broadleaf forests.

Warmer less warmer more colder more or colder less

Answers

Answer: Taiga forests are colder and receive less rainfall than temperate broadleaf.

Explanation: Taiga is very cold and most rainfall happens in summer.

Hope it helps

Which statement is true?

A. Waves carry objects as they travel through
water.

B. Waves don't carry things as they travel
through water.

Answers

Answer:

B. Waves don't carry things as they travel

through water.

Explanation:

The waves will not carry the raft in the direction the waves are moving. The waves will move the raft only up and down.

In what ways can the human-animal bond benefit human health?

Answers

Answer:

According to the Center for Disease Control (CDC), pet ownership can lower blood pressure and can positively influence our rate of physical activity. Our companions help us cope with stress which can improve cardiovascular health. For older adults, the human-animal bond has been directly linked to healthy aging.

Some _____ become memory cells when exposed to an infection agent and, if they encounter the same antigen in the future, they can respond quickly to destroy the invader before it causes illness.

Answers

Answer: T cells and B cells

Explanation:

Memory cells are the type of immune cells that responds to the previously exposed infectious agent. B and T cells become memory cells.

What are B and T cells?

B and T cells are antibody and cell-mediated immune cells respectively that are produced in response to the pathogens and fight the infectious agents that invade the body.

The cells differentiate into the same type and one in the form of the memory cell. The memory cells remember the infectious agent and respond quickly to the same antigen in a future invasion.

Therefore, B and T cells differentiate into memory cells.

Learn more about memory cells here:

https://brainly.com/question/19618460

Which gland is located closest to the human brain?.

Answers

The pituitary gland is the gland located closest to the human brain.

The diagram below represents the chemical pathway of a process in a human liver cell.A particular liver cell is unable to make substance C. One possible explanation for the inability of this cell to make substance C is that

Answers

Answer: nuclear DNA was altered resulting in the cell being unable to make enzyme Y.

Explanation:I don’t have an explanation I just took the test and got the answers

Water stress occurs when the demand for water exceeds the supply. This is likely to occur in:
Question 19 options:

Rainforest, small towns, industrial areas


Wet ecosystems, Sparsely populated Countries, less intense agriculture


Naturally arid ecosystems, Heavily populated countries, Areas with intensive, highly-irrigated agriculture

Tundras, medium populated countries, cattle-farming

Answers

Answer:

C. is the correct answer!

Explanation:

If its a naturally arid ecosystem then there wouldn't be that much water there in the first place.

The more people means the more supplies its needed to support those people, which can also lead to supply shortages such as in water.

Highly irrigated agriculture can also lead to tons of water being wasted, needed, and used up.

Why is dna replication considered a semi-conservative process.

Answers

Answer:

This process is said to be 'semiconservative' because one strand from each parent is conserved and remains intact after replication has taken place.

Answer:

Because each DNA strand is made up of one "conserved" old piece of DNA and one new piece.

Explanation:

during DNA replication once the two strands are unzipped on strand is older an theater strand is new

What structure is most likely used for motion

Answers

Answer:

Flagella.

Explanation:

Trust Me

The blood of the covenant is thicker than the water of the womb meaning:.

Answers

bonds that you've made by choice are more important than the people that you are bound to by the water of the womb

Examine the images here for two tree species. What best defines the taxonomy used to group these species?



Question 6 options:

These trees were grouped and named based on similar metabolic processes.


These trees were grouped and named based on shared evolutionary characteristics.


These trees were grouped and named based on overlapping geographic ranges.


These trees were grouped and named based on similar appearance.

Answers

These trees were grouped and named based on similar appearance.

The option of best defines the taxonomy used to group these species is that These trees were grouped and named based on shared evolutionary characteristics.

What is the taxonomy of a pine tree?

The  taxonomy Pinus is that it belongs to the following:

Kingdom : Plant Phylum : Coniferophyta Class : Pinopsida Order : Pinales Family : Pinaceae Genus : Pinus Species : Palustris P. MillThe  Common name: Longleaf pine.

Learn more about taxonomy from

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What does the wide variety of different types of animal eyes represent?.

Answers

Answer:

Their shape arose through convergent evolution, since that shape is beneficial for animals living in the ocean.

The ability of the ground to allow water to move through it into the groundwater, is a measure of the
the ground.
A. anchorage
B. permeability
C. Timing
D. waterlogging

Answers

Explanation:

water droplets are found in the pores between the sediment grains, which is porosity. When the water can travel between ores, that’s permeability.

Somatic and autonomic are divisions of the ______ nervous system.

Answers

Answer: The somatic and autonomic nervous systems are both part of the peripheral nervous system, which allows the brain and spinal cord to receive and send information to other areas of the body.    

   

Explanation:

1) With the worldwide demand for beef increasing each year, large areas of the Amazon rainforest are cleared for cattle grazing. Describe two ways that this practice contributes to Earth's greenhouse effect. Write your answer in complete sentences.​

Answers

Answer:

1. oxygen levels will decrease and carbon dioxide levels will increase due to the deforestation of trees.

2. increase of population= increase of land needed and more fossil fuels will be burnt .

Increase in CO2 levels due to deforestation of the trees in the forest will contribute to greenhouse effect.release of methane from cow and buffaloes reared for beef will also contribute to greenhouse effect.

What are the effects of clearing Amazon rainforest for beef ?Methane, a strong greenhouse gas, is produced during the digestion of plants and grasses by cows and other ruminant animals (such as goats and sheep). The term "enteric fermentation" refers to this process, which is where cow burps come from. Manure also releases methane into the air. Additionally, ruminant waste on pastures and chemical fertilizers applied to crops grown for bovine feed release nitrous oxide, another potent greenhouse gas.A more indirect but equally significant requirement for more land is the rise in cattle production. Cutting down trees to make room for new pastures releases carbon dioxide that has been stored in forests.

learn more about greenhouse effect here: https://brainly.com/question/11812392

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Why does the Sun rise earlier in New York City than it does in California?

(n0 us1ng g00gl3)

Answers

Answer:

bc its not the nearest state they Arnt near eachother i think lol srry if im wrong

Explanation:

which statement is true about electrically charged particles?

Answers

Answer:

protons are positive neutrons are neutral and electrons are negative

Explanation:

Hope this helps you!!!!!!!!! :D

Red imported fire ants are an ant species native to South America, but were introduced into the US in the 1950s.

a. True
b. False

Answers

This is (b.) False, because it was actually introduced in the 1930's.

what happens to the surface area to volume ratios as a cell gets larger?

Answers

Answer:

Smaller as the cell gets larger [Decrease]

Explanation:

The important point is that the surface area to the volume ratio gets smaller as the cell gets larger. Therefore, if the cell grows beyond a certain limit, not enough material will be able to cross the membrane fast enough to accommodate the increased cellular volume.

Assuming that the shape of the cell stays the same, if the cell increases it's linear dimension (length) by a factor of 2, the surface area will be increased by a factor of 4, and the volume will be increased by a factor of 8. You can verify that this is true with simple shapes such as spheres or cuboids.

The volume grows more rapidly than the surface area. Hence the surface area to volume ratio decreases.

In the process of ________, microbes detect the presence and density of other microbes and modify their metabolic activity in response.

Answers

ANSWER

In the process of Quorum Sensing, microbes detect the presence and density of other microbes and modify their metabolic activity in response.

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WatanabeHaruto

what makes rna and dna polymerase different? select all that apply.

Answers

Answer:

RNA polymerase is the main enzyme in RNA replication and has a lot more jobs than DNA polymerase. In DNA replication, DNA polymerase has helicase, ligase, primase and topoisomerase.

Explanation:


3. The air in the stratosphere does a lot of mixing because it is unstable.true or false

Answers

Answer: False

Explanation: air in the stratosphere is stable because warmer,less dense air sits over cooler, denser air. As a result there is little mixing of air within the layer.

Explain how Winogradsky columns illustrate the diversity of microorganisms found on Earth today in terms of the diversity of niches they occupy.

Answers

Answer:

How do Winogradsky columns illustrate the diversity of microorganisms found on Earth today in terms of the diversity of niches they occupy. Different layers in the column form based on the availability of oxygen and other nutrients. ... A large diversity of microbes are adapted to life without oxygen.

what results from sea-floor spreading? please help

Answers

Answer:

The crust cracking.

Explanation:

Seafloor spreading is a geologic process in which tectonic plates—large slabs of Earth's lithosphere—split apart from each other. ... The less-dense material rises, often forming a mountain or elevated area of the seafloor.

Answer:

Seafloor spreading creates new oceanic crust at a mid-ocean ridge.

Explanation:

When this new material reaches the end of the plate and comes into contact with another plate, whether continental or not, a convergent or a transform boundary will occur.

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Can someone please help me

Answers

Please select the best answer from the choices provided.

When flexing all of the fingers joints maximally in combination with maximal wrist flexion and no further flexion of any of the involved joints may be reached, the result is _______.

Answers

When no further flexion of any of the involved joints may be reached, the result is an active insufficiency of the extensor muscles. They are involved in muscle extension.

What are the extensor muscles?

The extensor muscles are muscles capable of increasing the angle between members in a given limb.

Extensor muscles include, for example, teres major, the latissimus dorsi, triceps brachii, etc.

Moreover, flexor muscles include upper limb flexors such as biceps brachii and brachialis.

Learn more about the extensor muscles here:

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can i get some help with this question

Answers

Answer:

photograph cells in mitosis

Answer:

B. i think im not 100% sure

Explanation:

Im what type of organism does cellular respiration occur?

Answers

Answer:

this occurs in animals and plants they use oxygen molecules to break down food it also occurs in cells of animals, plants, and fungi, and also in algae and other protists.

Explanation:

Hope it helps brainly 4 effort? bless u btw

What do the fungus that causes athlete's foot, the tick that spreads Lyme disease, and body lice all have in common

Answers

The common difference between them is that they are all bacteria.
Hope it’s helps
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