The DNA sequence of the template or the bottom strand of an mRNA gene is 5-CCGGAATT-3. The sequence of the transcript will be 3-GGCCUUAA-5.
The base sequence in DNA and mRNA are complementary, and their alignment results in the production of mRNA. DNA bases A, T, G, and C pair with RNA bases U, A, C, and G, respectively, the sequence of mRNA is determined by the base pairing of RNA nucleotides with the DNA template strand. RNA is synthesized by complementary base pairing with the DNA strand in a process known as transcription.
DNA is made up of nucleotides with a sugar-phosphate backbone and a nitrogenous base, the nitrogenous base can be one of four nucleotides: adenine, guanine, cytosine, and thymine. In DNA, thymine is used in place of uracil, which is used in RNA. The complementary RNA strand is then synthesized by matching up the RNA nucleotides to the DNA template strand, this is what determines the sequence of mRNA. So therefore the sequence of the transcript in the given DNA template strand is 3-GGCCUUAA-5.
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